abstract：:The filamentous cyanobacterium Anabaena (Nostoc) sp. strain PCC 7120 produces specialized cells for nitrogen fixation called heterocysts. Previous work showed that the group 2 sigma factor sigE (alr4249; previously called sigF) is upregulated in differentiating heterocysts 16 h after nitrogen step-down. We now show th...

journal_title：Journal of bacteriology

authors： Mella-Herrera RA,Neunuebel MR,Kumar K,Saha SK,Golden JW

更新日期：2011-04-01 00:00:00

abstract：:Campylobacter jejuni remains among the leading causes of bacterial food-borne illness. The current understanding of Campylobacter physiology suggests that it is asaccharolytic and is unable to catabolize exogenous carbohydrates. Contrary to this paradigm, we provide evidence for l-fucose utilization by C. jejuni. The ...

journal_title：Journal of bacteriology

authors： Muraoka WT,Zhang Q

更新日期：2011-03-01 00:00:00

abstract：:The function of the essential inner membrane protein (IMP) YidC in Escherichia coli has been studied for a limited number of model IMPs and primarily using targeted approaches. These studies suggested that YidC acts at the level of insertion, folding, and quality control of IMPs, both in the context of the Sec translo...

journal_title：Journal of bacteriology

authors： Yu Z,Bekker M,Tramonti A,Cook GM,van Ulsen P,Scheffers DJ,de Mattos JT,De Biase D,Luirink J

更新日期：2011-03-01 00:00:00

abstract：:The fucose-/mannose-specific lectin LecB from Pseudomonas aeruginosa is transported to the outer membrane; however, the mechanism used is not known so far. Here, we report that LecB is present in the periplasm of P. aeruginosa in two variants of different sizes. Both were functional and could be purified by their affi...

journal_title：Journal of bacteriology

authors： Bartels KM,Funken H,Knapp A,Brocker M,Bott M,Wilhelm S,Jaeger KE,Rosenau F

更新日期：2011-03-01 00:00:00

abstract：:Riemerella anatipestifer is a Gram-negative, rod-shaped bacterium associated with epizootic infections in poultry. R. anatipestifer strain RA-YM, belonging to the serotype 1 prevalent in China, is a clinically isolated strain with high-level virulence. Here, we report the first genome sequence of this species. ...

journal_title：Journal of bacteriology

authors： Zhou Z,Peng X,Xiao Y,Wang X,Guo Z,Zhu L,Liu M,Jin H,Bi D,Li Z,Sun M

更新日期：2011-03-01 00:00:00

abstract：:Sulfolobus acidocaldarius utilizes glucose and xylose as sole carbon sources, but its ability to metabolize these sugars simultaneously is not known. We report the absence of diauxie during growth of S. acidocaldarius on glucose and xylose as co-carbon sources. The presence of glucose did not repress xylose utilizatio...

journal_title：Journal of bacteriology

authors： Joshua CJ,Dahl R,Benke PI,Keasling JD

更新日期：2011-03-01 00:00:00

abstract：:Escherichia coli exhibits a wide range of lifestyles encompassing commensalism and various pathogenic behaviors which its highly dynamic genome contributes to develop. How environmental and host factors shape the genetic structure of E. coli strains remains, however, largely unknown. Following a previous study of E. c...

journal_title：Journal of bacteriology

authors： Vieira G,Sabarly V,Bourguignon PY,Durot M,Le Fèvre F,Mornico D,Vallenet D,Bouvet O,Denamur E,Schachter V,Médigue C

更新日期：2011-03-01 00:00:00

abstract：:Achromobacter xylosoxidans strain A8 was isolated from soil contaminated with polychlorinated biphenyls. It can use 2-chlorobenzoate and 2,5-dichlorobenzoate as sole sources of carbon and energy. This property makes it a good starting microorganism for further development toward a bioremediation tool. The genome of A....

journal_title：Journal of bacteriology

authors： Strnad H,Ridl J,Paces J,Kolar M,Vlcek C,Paces V

更新日期：2011-02-01 00:00:00

abstract：:Transcriptome analysis of a Bacillus subtilis rok strain that showed reduced complex colony structure formation revealed significant downregulation of the yuaB gene. Overexpression of yuaB restored structure formation in the rok strain. We show that transcription of yuaB is indirectly regulated by Rok, independently f...

journal_title：Journal of bacteriology

authors： Kovács AT,Kuipers OP

更新日期：2011-02-01 00:00:00

abstract：:DifA is a methyl-accepting chemotaxis protein (MCP)-like sensory transducer that regulates exopolysaccharide (EPS) production in Myxococcus xanthus. Here mutational analysis and molecular biology were used to probe the signaling mechanisms of DifA in EPS regulation. We first identified the start codon of DifA experime...

journal_title：Journal of bacteriology

authors： Xu Q,Black WP,Nascimi HM,Yang Z

更新日期：2011-02-01 00:00:00

abstract：:Comparative genome analysis of Enterococcus faecium recently revealed that a genomic island containing the esp gene, referred to as the esp-containing pathogenicity island (esp PAI), can be transferred by conjugation and contains a partial Tn916-like element and an integrase gene, intA. Here, we characterize the role ...

journal_title：Journal of bacteriology

authors： Top J,Sinnige JC,Majoor EA,Bonten MJ,Willems RJ,van Schaik W

更新日期：2011-02-01 00:00:00

abstract：:Application of in vivo expression technology (IVET) to Yersinia ruckeri, an important fish pathogen, allowed the identification of two adjacent genes that represent a novel bacterial system involved in the uptake and degradation of l-cysteine. Analysis of the translational products of both genes showed permease domain...

journal_title：Journal of bacteriology

authors： Méndez J,Reimundo P,Pérez-Pascual D,Navais R,Gómez E,Guijarro JA

更新日期：2011-02-01 00:00:00

abstract：:The O antigen is the outer part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria and contains many repeats of an oligosaccharide unit. It contributes to antigenic variability and is essential to the full function and virulence of bacteria. Shigella is a Gram-negative human pathogen that ...

journal_title：Journal of bacteriology

authors： Xu C,Liu B,Hu B,Han Y,Feng L,Allingham JS,Szarek WA,Wang L,Brockhausen I

更新日期：2011-01-01 00:00:00

abstract：:Escherichia coli UM146 was isolated from the ileum of a Crohn's disease patient. It adheres to and invades enterocytes and can replicate inside macrophages. Its complete genome sequence reveals that it is most closely related to the human urinary tract pathogen E. coli CFT073, but it has a host of genes that are novel...

journal_title：Journal of bacteriology

authors： Krause DO,Little AC,Dowd SE,Bernstein CN

更新日期：2011-01-01 00:00:00

abstract：:Many streptococcal pathogens require a polysaccharide capsule for survival in the host during systemic infection. The highly conserved CpsA protein is proposed to be a transcriptional regulator of capsule production in streptococci, although the regulatory mechanism is unknown. Hydropathy plots of CpsA predict an inte...

journal_title：Journal of bacteriology

authors： Hanson BR,Lowe BA,Neely MN

更新日期：2011-01-01 00:00:00

abstract：:Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate stress response. The transcription factor SgrR activates transcription of the small RNA SgrS under stress conditions. SgrS represses translatio...

journal_title：Journal of bacteriology

authors： Sun Y,Vanderpool CK

更新日期：2011-01-01 00:00:00

abstract：:The Bacillus anthracis endospore loses resistance properties during germination when its cortex peptidoglycan is degraded by germination-specific lytic enzymes (GSLEs). Although this event normally employs several GSLEs for complete cortex removal, the SleB protein alone can facilitate enough cortex hydrolysis to prod...

journal_title：Journal of bacteriology

authors： Heffron JD,Sherry N,Popham DL

更新日期：2011-01-01 00:00:00

abstract：:The Haemophilus cryptic genospecies is an important cause of maternal genital tract and neonatal systemic infections and initiates infection by colonizing the genital or respiratory epithelium. In recent work, we identified a unique Haemophilus cryptic genospecies protein called Cha, which mediates efficient adherence...

journal_title：Journal of bacteriology

authors： Sheets AJ,St Geme JW 3rd

更新日期：2011-01-01 00:00:00

abstract：:The Escherichia coli rluD gene encodes a pseudouridine synthase responsible for the pseudouridine (Ψ) modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA. It has been reported that deletion of rluD in K-12 strains of E. coli is associated with extremely slow growth, increased readthrough of stop co...

journal_title：Journal of bacteriology

authors： O'Connor M,Gregory ST

更新日期：2011-01-01 00:00:00

abstract：:The Staphylococcus aureus autoinducer-2 (AI-2) producer protein LuxS is phosphorylated by the Ser/Thr kinase Stk1 at a unique position, Thr14. The enzymatic activity of the phosphorylated isoform of LuxS was abrogated compared to that of nonphosphorylated LuxS, thus providing the first evidence of an AI-2-producing en...

journal_title：Journal of bacteriology

authors： Cluzel ME,Zanella-Cléon I,Cozzone AJ,Fütterer K,Duclos B,Molle V

更新日期：2010-12-01 00:00:00

abstract：:Upon exposure to alkylating agents, Escherichia coli increases expression of aidB along with three genes (ada, alkA, and alkB) that encode DNA repair proteins. While the biological roles of the Ada, AlkA, and AlkB proteins have been defined, despite many efforts, the molecular functions of AidB remain largely unknown....

journal_title：Journal of bacteriology

authors： Rippa V,Amoresano A,Esposito C,Landini P,Volkert M,Duilio A

更新日期：2010-12-01 00:00:00

abstract：:Plasmids pRAS3.1 and pRAS3.2 are natural variants of the IncQ-2 plasmid family, that except for two differences, have identical plasmid backbones. Plasmid pRAS3.1 has four 22-bp iterons in its oriV region, while pRAS3.2 has only three 6-bp repeats and pRAS3.1 has five 6-bp repeats in the promoter region of the mobB-mo...

journal_title：Journal of bacteriology

authors： Loftie-Eaton W,Rawlings DE

更新日期：2010-12-01 00:00:00

abstract：:The RpoS sigma factor (σ(S)) is the master regulator of the bacterial response to a variety of stresses. Mutants in rpoS arise in bacterial populations in the absence of stress, probably as a consequence of a subtle balance between self-preservation and nutritional competence. We characterized here one natural rpoS mu...

journal_title：Journal of bacteriology

authors： Monteil V,Kolb A,Mayer C,Hoos S,England P,Norel F

更新日期：2010-12-01 00:00:00

abstract：:Molybdenum (Mo) is an important trace element that is toxic at high concentrations. To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis. The insertion sites of six independent isolates mapped within the s...

journal_title：Journal of bacteriology

authors： Gisin J,Müller A,Pfänder Y,Leimkühler S,Narberhaus F,Masepohl B

更新日期：2010-11-01 00:00:00

abstract：:The Staphylococcus aureus SarA global regulator controls the expression of numerous virulence genes, often in conjunction with the agr quorum-sensing system and its effector RNA, RNAIII. In the present study, we have examined the role of both SarA and RNAIII on the regulation of the promoter of tst, encoding staphyloc...

journal_title：Journal of bacteriology

authors： Andrey DO,Renzoni A,Monod A,Lew DP,Cheung AL,Kelley WL

更新日期：2010-11-01 00:00:00

abstract：:Here we show that the type III secretion gatekeeper protein SepL resembles an aberrant effector protein in binding to a class 1 type III secretion chaperone (Orf12, here renamed CesL). We also show that short N-terminal fragments (≤70 amino acids) from SepL are capable of targeting fusion proteins for secretion and tr...

journal_title：Journal of bacteriology

authors： Younis R,Bingle LE,Rollauer S,Munera D,Busby SJ,Johnson S,Deane JE,Lea SM,Frankel G,Pallen MJ

更新日期：2010-11-01 00:00:00

abstract：:In contrast to canonical phage endolysins, which require holin-mediated disruption of the membrane to gain access to attack the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where they accumulate in an inactive form tethered to the membrane by their N-terminal SAR domains. SAR ...

journal_title：Journal of bacteriology

authors： Kuty GF,Xu M,Struck DK,Summer EJ,Young R

更新日期：2010-11-01 00:00:00

abstract：:The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...

journal_title：Journal of bacteriology

authors： Geoghegan JA,Corrigan RM,Gruszka DT,Speziale P,O'Gara JP,Potts JR,Foster TJ

更新日期：2010-11-01 00:00:00

abstract：:We report the cloning and characterization of the biosynthetic gene cluster (crtE, crtB, crtI, crtE2, crtYg, crtYh, and crtX) of the γ-cyclic C(50) carotenoid sarcinaxanthin in Micrococcus luteus NCTC2665. Expression of the complete and partial gene cluster in Escherichia coli hosts revealed that sarcinaxanthin biosyn...

journal_title：Journal of bacteriology

authors： Netzer R,Stafsnes MH,Andreassen T,Goksøyr A,Bruheim P,Brautaset T

更新日期：2010-11-01 00:00:00

abstract：:Salmonella enterica degrades 1,2-propanediol (1,2-PD) in a coenzyme B12 (adenosylcobalamin, AdoCbl)-dependent fashion. Salmonella obtains AdoCbl by assimilation of complex precursors, such as vitamin B12 and hydroxocobalamin. Assimilation of these compounds requires reduction of their central cobalt atom from Co3+ to ...

journal_title：Journal of bacteriology

authors： Cheng S,Bobik TA

更新日期：2010-10-01 00:00:00

abstract：:Using an oligonucleotide microarray, we searched for previously unrecognized transcription units in intergenic regions in the genome of Bacillus subtilis, with an emphasis on identifying small genes activated during spore formation. Nineteen transcription units were identified, 11 of which were shown to depend on one ...

journal_title：Journal of bacteriology

authors： Schmalisch M,Maiques E,Nikolov L,Camp AH,Chevreux B,Muffler A,Rodriguez S,Perkins J,Losick R

更新日期：2010-10-01 00:00:00

abstract：:In this study, we identified and characterized the enzymatic properties of MG_186, a calcium-dependent Mycoplasma genitalium nuclease. MG_186 displays the hallmarks of nucleases, as indicated by its amino acid sequence similarity to other nucleases. We cloned, UGA corrected, expressed, purified, and demonstrated that ...

journal_title：Journal of bacteriology

authors： Li L,Krishnan M,Baseman JB,Kannan TR

更新日期：2010-10-01 00:00:00

abstract：:Cell division in Caulobacter crescentus involves constriction and fission of the inner membrane (IM) followed about 20 min later by fission of the outer membrane (OM) and daughter cell separation. In contrast to Escherichia coli, the Caulobacter Tol-Pal complex is essential. Cryo-electron microscopy images of the Caul...

journal_title：Journal of bacteriology

authors： Yeh YC,Comolli LR,Downing KH,Shapiro L,McAdams HH

更新日期：2010-10-01 00:00:00

abstract：:An extremely thermophilic bacterium, Thermus thermophilus HB8, is one of the model organisms for systems biology. Its genome consists of a chromosome (1.85 Mb), a megaplasmid (0.26 Mb) designated pTT27, and a plasmid (9.3 kb) designated pTT8, and the complete sequence is available. We show here that T. thermophilus is...

journal_title：Journal of bacteriology

authors： Ohtani N,Tomita M,Itaya M

更新日期：2010-10-01 00:00:00

abstract：:The delta subunit of RNA polymerase, RpoE, is widespread in low-G+C Gram-positive bacteria and is thought to play a role in enhancing transcriptional specificity by blocking RNA polymerase binding at weak promoter sites and stimulating RNA synthesis by accelerating core enzyme recycling. Despite the well-studied bioch...

journal_title：Journal of bacteriology

authors： Xue X,Tomasch J,Sztajer H,Wagner-Döbler I

更新日期：2010-10-01 00:00:00

abstract：:The DosS (DevS) and DosT histidine kinases form a two-component system together with the DosR (DevR) response regulator in Mycobacterium tuberculosis. DosS and DosT, which have high sequence similarity to each other over the length of their amino acid sequences, contain two GAF domains (GAF-A and GAF-B) in their N-ter...

journal_title：Journal of bacteriology

authors： Kim MJ,Park KJ,Ko IJ,Kim YM,Oh JI

更新日期：2010-10-01 00:00:00

abstract：:Many genomes of anaerobic hyperthermophiles encode multiple homologs of NAD(P)H oxidase that are thought to function in response to oxidative stress. We investigated one of the seven NAD(P)H oxidase homologs (TK1481) in the sulfur-reducing hyperthermophilic archaeon Thermococcus kodakarensis, focusing on the catalytic...

journal_title：Journal of bacteriology

authors： Kobori H,Ogino M,Orita I,Nakamura S,Imanaka T,Fukui T

更新日期：2010-10-01 00:00:00

abstract：:Acclimation to high salt concentrations involves concerted changes in gene expression. For the majority of salt-regulated genes, the mechanism underlying the induction process is not known. The gene ggpS (sll1566), which encodes the glucosylglycerol-phosphate synthase responsible for the synthesis of the compatible so...

journal_title：Journal of bacteriology

authors： Klähn S,Höhne A,Simon E,Hagemann M

更新日期：2010-09-01 00:00:00

abstract：:Halalkalicoccus jeotgali B3(T), isolated from salt-fermented seafood from South Korea, is an extremely halophilic archaeon belonging to the family Halobacteriaceae. Here, we present the complete genome sequence of the type strain H. jeotgali B3(T) (3,698,650 bp, with a G+C content of 62.5%), which consists of one chro...

journal_title：Journal of bacteriology

authors： Roh SW,Nam YD,Nam SH,Choi SH,Park HS,Bae JW

更新日期：2010-09-01 00:00:00

abstract：:The ferric enterobactin (FeEnt) receptor CfrA is present in the majority of Campylobacter jejuni isolates and is responsible for high-affinity iron acquisition. Our recent work and that of others strongly suggested the existence of another FeEnt uptake system in Campylobacter. Here we have identified and characterized...

journal_title：Journal of bacteriology

authors： Xu F,Zeng X,Haigh RD,Ketley JM,Lin J

更新日期：2010-09-01 00:00:00